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1. Mix measured amount
of AlgaMac-3000 with either fresh or saltwater in
electric blender for one minute or until completely emulsified.
Spray-dried particles break down
into individual cells (6-8 microns).
2. When blending is complete,
strain through sub-80 micron mesh to eliminate any foam.
Transfer newly hatched Artemia nauplii to an enrichment tank with clean, filtered seawater. Maximum density of Artemia should not exceed 100,000 nauplii per liter of water. Add the prepared
AlgaMac-3000 mixture to the enrichment tank at a rate of 0.2 grams per liter (per 100,000 nauplii). Aerate enrichment tank vigorously so that oxygen levels exceed 4ppm during the
8 hours of enrichment. Harvest the enriched Artemia nauplii after
8 hours. If higher levels of DHA
are desired, repeat enrichment process by adding another
0.2 grams per liter for an additional 8 hours.
Ratio of Application: 0.2 grams /
liter / 100,000 nauplii / 8 hour enrichment period
Enrichment for Rotifers
ROTIFER ENRICHMENT PREPARATION
1. Hydrate dry ALGAMAC-3000 powder in fresh or saltwater (preferably fresh) and mix in a blender for
one minute or until completely emulsified. Up to 30 grams of dry
product per liter may be emulsified at one time.
- 2. Strain off any excess surface foam. The mixture is now ready for use as indicated below.
ALGAMAC-3000 prepared by this procedure will produce particle sizes of under 10 microns.
- 3. Transfer harvested rotifers to an enrichment tank with clean, filtered seawater. Maximum density of rotifers should not exceed 500,000 rotifers per liter of seawater. Add the prepared ALGAMAC-3000 mixture to the enrichment tank at a rate of 300 mg per 1,000,000 rotifers. Aerate vigorously so that oxygen levels exceed 4.0 ppm during the
8 hours of enrichment. If higher levels of DHA are desired, repeat enrichment process by adding another 300 mg ALGAMAC-3000 per 1,000,000 rotifers for an additional
Ratio of Application: 300 mg ALGAMAC-3000 per 1,000,000 rotifers.
Please note: The
applications rate is only a suggested formula and users should
conduct their own optimization rates for AlgaMac-3000 since it
contains higher levels of DHA than the AlgaMac-2000 product.
IMPORTANT FEEDING NOTE:
When enriching with
AlgaMac-3000, continue to feed the rotifers 35% of the normal
application of live algae and/or culture diets normally used. This
will provide sufficient proteins and carbohydrates for proper
metabolism during the enrichment period.
symptoms of dysfunctional metabolilsm due to high fat enrichment are
lethargic activity of the rotifers at the 5-6 hour after enrichment
begins. With the continued application of live algae or culture
diets at one third normal feeding rates, the rotifers will uptake
the enrichment properly."