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Allow the Aquafauna refractometer to rest for approximately one hour at 20 degrees Celsius (68 deg F) or in the ambient temperature in which you will be taking readings. Place distilled water under the daylight plate. The boundary refraction line should read 1.000 specific gravity or 0 ppt. If line is above of below the 1.000 specific gravity / 0 ppt marking on the scale, remove the plastic cap (new models have no cap), which has been sealed with silicone. Then, while looking through unit, use a small screwdriver to turn adjustment screw counter clockwise until boundary refraction line moves above zero then turn the adjustment screw clockwise until refraction line moves down to zero. Always make your "zero adjustments" in the clockwise direction so as to prevent any thread slippage. When line is at zero, place a small drop of silicone sealant on top of adjustment screw (not necessary on new models) and replace the plastic cap. With finger, press center of plastic cap and twist cap half turn to help spread sealant and insure a watertight seal.